Bacterial diversity associated with poplar trees grown on a Hg-contaminated site: Community characterization and isolation of Hg-resistant plant growth-promoting bacteria.
Identifieur interne : 001034 ( Main/Exploration ); précédent : 001033; suivant : 001035Bacterial diversity associated with poplar trees grown on a Hg-contaminated site: Community characterization and isolation of Hg-resistant plant growth-promoting bacteria.
Auteurs : Alexis Durand [France] ; François Maillard [France] ; Vanessa Alvarez-Lopez [France] ; Sarah Guinchard [France] ; Coralie Bertheau [France] ; Benoit Valot [France] ; Damien Blaudez [France] ; Michel Chalot [France]Source :
- The Science of the total environment [ 1879-1026 ] ; 2018.
Descripteurs français
- KwdFr :
- Bactéries (classification), Bactéries (métabolisme), Dépollution biologique de l'environnement (MeSH), Développement des plantes (MeSH), Mercure (analyse), Mercure (métabolisme), Microbiologie du sol (MeSH), Polluants du sol (analyse), Polluants du sol (métabolisme), Populus (croissance et développement), Rhizosphère (MeSH), Surveillance de l'environnement (MeSH).
- MESH :
- analyse : Mercure, Polluants du sol.
- croissance et développement : Populus.
- métabolisme : Bactéries, Mercure, Polluants du sol.
- classification : Bactéries, Dépollution biologique de l'environnement, Développement des plantes, Microbiologie du sol, Rhizosphère, Surveillance de l'environnement.
English descriptors
- KwdEn :
- Bacteria (classification), Bacteria (metabolism), Biodegradation, Environmental (MeSH), Environmental Monitoring (MeSH), Mercury (analysis), Mercury (metabolism), Plant Development (MeSH), Populus (growth & development), Rhizosphere (MeSH), Soil Microbiology (MeSH), Soil Pollutants (analysis), Soil Pollutants (metabolism).
- MESH :
- chemical , analysis : Mercury, Soil Pollutants.
- classification : Bacteria.
- growth & development : Populus.
- metabolism : Bacteria, Mercury, Soil Pollutants.
- Biodegradation, Environmental, Environmental Monitoring, Plant Development, Rhizosphere, Soil Microbiology.
Abstract
Industrial waste dumps are rarely colonized by vegetation after they have been abandoned, indicating biological infertility. Revegetation of industrial tailings dumps is thus necessary to prevent wind erosion, metal leaching and has been shown to restore soil functions and ecosystem services. However, little is known about the microbial colonization and community structure of vegetated tailings following the application of restoration technologies. In this study, we investigated the rhizosphere and phyllosphere bacterial communities of a poplar tree plantation within a phytomanagement-based restoration program of a Hg-contaminated site. We used Illumina-based sequencing combined with culture-dependent approaches to describe plant-associated bacterial communities and to isolate growth-promoting bacteria (PGPB) and Hg-resistant bacteria. The genus Streptomyces was highly specific to the root community, accounting for 24.4% of the relative abundance but only representing 0.8% of the soil community, whereas OTUs from the Chloroflexi phylum were essentially detected in the soil community. Aboveground habitats were dominated by bacteria from the Deinococcus-Thermus phylum, which were not detected in belowground habitats. Leaf and stem habitats were characterized by several dominant OTUs, such as those from the phylum Firmicutes in the stems or from the genera Methylobacterium, Kineococcus, Sphingomonas and Hymenobacter in the leaves. Belowground habitats hosted more cultivable Hg-resistant bacteria than aboveground habitats and more Hg-resistant bacteria were found on the episphere than in endospheric habitats. Hg-resistant isolates exhibiting plant growth-promoting (PGP) traits, when used as inoculants of Capsicum annuum, were shown to increase its root dry biomass but not Hg concentration. The N2-fixing and Hg-resistant species Pseudomonas graminis, observed in the poplar phyllosphere, may be a key microorganism for the restoration of industrial tailings dumps.
DOI: 10.1016/j.scitotenv.2017.12.069
PubMed: 29890585
Affiliations:
- France
- Bourgogne-Franche-Comté, Franche-Comté, Grand Est, Lorraine (région)
- Montbéliard, Vandœuvre-lès-Nancy
- Université de Lorraine
Links toward previous steps (curation, corpus...)
Le document en format XML
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Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard</wicri:regionArea><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Franche-Comté</region><settlement type="city">Montbéliard</settlement></placeName></affiliation></author><author><name sortKey="Maillard, Francois" sort="Maillard, Francois" uniqKey="Maillard F" first="François" last="Maillard">François Maillard</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard</wicri:regionArea><placeName><region type="region" 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type="city">Montbéliard</settlement></placeName></affiliation></author><author><name sortKey="Guinchard, Sarah" sort="Guinchard, Sarah" uniqKey="Guinchard S" first="Sarah" last="Guinchard">Sarah Guinchard</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard</wicri:regionArea><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Franche-Comté</region><settlement type="city">Montbéliard</settlement></placeName></affiliation></author><author><name sortKey="Bertheau, Coralie" sort="Bertheau, Coralie" uniqKey="Bertheau C" first="Coralie" last="Bertheau">Coralie Bertheau</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard</wicri:regionArea><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Franche-Comté</region><settlement type="city">Montbéliard</settlement></placeName></affiliation></author><author><name sortKey="Valot, Benoit" sort="Valot, Benoit" uniqKey="Valot B" first="Benoit" last="Valot">Benoit Valot</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard</wicri:regionArea><placeName><region type="region" nuts="2">Bourgogne-Franche-Comté</region><region type="old region" nuts="2">Franche-Comté</region><settlement type="city">Montbéliard</settlement></placeName></affiliation></author><author><name sortKey="Blaudez, Damien" sort="Blaudez, Damien" uniqKey="Blaudez D" first="Damien" last="Blaudez">Damien Blaudez</name><affiliation wicri:level="4"><nlm:affiliation>Université de Lorraine, UMR CNRS 7360 Laboratoire Interdisciplinaire des Environnements Continentaux, Faculté des Sciences et Technologies, BP 70239, 54506, Vandœuvre-lès-Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, UMR CNRS 7360 Laboratoire Interdisciplinaire des Environnements Continentaux, Faculté des Sciences et Technologies, BP 70239, 54506, Vandœuvre-lès-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Chalot, Michel" sort="Chalot, Michel" uniqKey="Chalot M" first="Michel" last="Chalot">Michel Chalot</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France; Université de Lorraine, Faculté des Sciences et Technologies, BP 70239, 54506 Vandoeuvre-les-Nancy, France. Electronic address: michel.chalot@univ-fcomte.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France; Université de Lorraine, Faculté des Sciences et Technologies, BP 70239, 54506 Vandoeuvre-les-Nancy</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Vandœuvre-lès-Nancy</settlement></placeName></affiliation></author></analytic><series><title level="j">The Science of the total environment</title><idno type="eISSN">1879-1026</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (classification)</term><term>Bacteria (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Environmental Monitoring (MeSH)</term><term>Mercury (analysis)</term><term>Mercury (metabolism)</term><term>Plant Development (MeSH)</term><term>Populus (growth & development)</term><term>Rhizosphere (MeSH)</term><term>Soil Microbiology (MeSH)</term><term>Soil Pollutants (analysis)</term><term>Soil Pollutants (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bactéries (classification)</term><term>Bactéries (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Développement des plantes (MeSH)</term><term>Mercure (analyse)</term><term>Mercure (métabolisme)</term><term>Microbiologie du sol (MeSH)</term><term>Polluants du sol (analyse)</term><term>Polluants du sol (métabolisme)</term><term>Populus (croissance et développement)</term><term>Rhizosphère (MeSH)</term><term>Surveillance de l'environnement (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Mercury</term><term>Soil Pollutants</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Mercure</term><term>Polluants du sol</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacteria</term><term>Mercury</term><term>Soil Pollutants</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bactéries</term><term>Mercure</term><term>Polluants du sol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Environmental Monitoring</term><term>Plant Development</term><term>Rhizosphere</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Bactéries</term><term>Dépollution biologique de l'environnement</term><term>Développement des plantes</term><term>Microbiologie du sol</term><term>Rhizosphère</term><term>Surveillance de l'environnement</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Industrial waste dumps are rarely colonized by vegetation after they have been abandoned, indicating biological infertility. Revegetation of industrial tailings dumps is thus necessary to prevent wind erosion, metal leaching and has been shown to restore soil functions and ecosystem services. However, little is known about the microbial colonization and community structure of vegetated tailings following the application of restoration technologies. In this study, we investigated the rhizosphere and phyllosphere bacterial communities of a poplar tree plantation within a phytomanagement-based restoration program of a Hg-contaminated site. We used Illumina-based sequencing combined with culture-dependent approaches to describe plant-associated bacterial communities and to isolate growth-promoting bacteria (PGPB) and Hg-resistant bacteria. The genus Streptomyces was highly specific to the root community, accounting for 24.4% of the relative abundance but only representing 0.8% of the soil community, whereas OTUs from the Chloroflexi phylum were essentially detected in the soil community. Aboveground habitats were dominated by bacteria from the Deinococcus-Thermus phylum, which were not detected in belowground habitats. Leaf and stem habitats were characterized by several dominant OTUs, such as those from the phylum Firmicutes in the stems or from the genera Methylobacterium, Kineococcus, Sphingomonas and Hymenobacter in the leaves. Belowground habitats hosted more cultivable Hg-resistant bacteria than aboveground habitats and more Hg-resistant bacteria were found on the episphere than in endospheric habitats. Hg-resistant isolates exhibiting plant growth-promoting (PGP) traits, when used as inoculants of Capsicum annuum, were shown to increase its root dry biomass but not Hg concentration. The N<sub>2</sub>-fixing and Hg-resistant species Pseudomonas graminis, observed in the poplar phyllosphere, may be a key microorganism for the restoration of industrial tailings dumps.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29890585</PMID><DateCompleted><Year>2018</Year><Month>06</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>06</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1026</ISSN><JournalIssue CitedMedium="Internet"><Volume>622-623</Volume><PubDate><Year>2018</Year><Month>May</Month><Day>01</Day></PubDate></JournalIssue><Title>The Science of the total environment</Title><ISOAbbreviation>Sci Total Environ</ISOAbbreviation></Journal><ArticleTitle>Bacterial diversity associated with poplar trees grown on a Hg-contaminated site: Community characterization and isolation of Hg-resistant plant growth-promoting bacteria.</ArticleTitle><Pagination><MedlinePgn>1165-1177</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0048-9697(17)33488-5</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.scitotenv.2017.12.069</ELocationID><Abstract><AbstractText>Industrial waste dumps are rarely colonized by vegetation after they have been abandoned, indicating biological infertility. Revegetation of industrial tailings dumps is thus necessary to prevent wind erosion, metal leaching and has been shown to restore soil functions and ecosystem services. However, little is known about the microbial colonization and community structure of vegetated tailings following the application of restoration technologies. In this study, we investigated the rhizosphere and phyllosphere bacterial communities of a poplar tree plantation within a phytomanagement-based restoration program of a Hg-contaminated site. We used Illumina-based sequencing combined with culture-dependent approaches to describe plant-associated bacterial communities and to isolate growth-promoting bacteria (PGPB) and Hg-resistant bacteria. The genus Streptomyces was highly specific to the root community, accounting for 24.4% of the relative abundance but only representing 0.8% of the soil community, whereas OTUs from the Chloroflexi phylum were essentially detected in the soil community. Aboveground habitats were dominated by bacteria from the Deinococcus-Thermus phylum, which were not detected in belowground habitats. Leaf and stem habitats were characterized by several dominant OTUs, such as those from the phylum Firmicutes in the stems or from the genera Methylobacterium, Kineococcus, Sphingomonas and Hymenobacter in the leaves. Belowground habitats hosted more cultivable Hg-resistant bacteria than aboveground habitats and more Hg-resistant bacteria were found on the episphere than in endospheric habitats. Hg-resistant isolates exhibiting plant growth-promoting (PGP) traits, when used as inoculants of Capsicum annuum, were shown to increase its root dry biomass but not Hg concentration. The N<sub>2</sub>-fixing and Hg-resistant species Pseudomonas graminis, observed in the poplar phyllosphere, may be a key microorganism for the restoration of industrial tailings dumps.</AbstractText><CopyrightInformation>Copyright © 2017 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Durand</LastName><ForeName>Alexis</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maillard</LastName><ForeName>François</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alvarez-Lopez</LastName><ForeName>Vanessa</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guinchard</LastName><ForeName>Sarah</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bertheau</LastName><ForeName>Coralie</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Valot</LastName><ForeName>Benoit</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Blaudez</LastName><ForeName>Damien</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Université de Lorraine, UMR CNRS 7360 Laboratoire Interdisciplinaire des Environnements Continentaux, Faculté des Sciences et Technologies, BP 70239, 54506, Vandœuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chalot</LastName><ForeName>Michel</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire Chrono-Environnement, UMR 6249, Université de Bourgogne Franche-Comté, Pôle Universitaire du Pays de Montbéliard, 4 place Tharradin, BP 71427, 25211 Montbéliard, France; Université de Lorraine, Faculté des Sciences et Technologies, BP 70239, 54506 Vandoeuvre-les-Nancy, France. Electronic address: michel.chalot@univ-fcomte.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Sci Total Environ</MedlineTA><NlmUniqueID>0330500</NlmUniqueID><ISSNLinking>0048-9697</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>FXS1BY2PGL</RegistryNumber><NameOfSubstance UI="D008628">Mercury</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004784" MajorTopicYN="Y">Environmental Monitoring</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008628" MajorTopicYN="N">Mercury</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D063245" MajorTopicYN="N">Plant Development</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058441" MajorTopicYN="N">Rhizosphere</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Mercury-resistant bacteria</Keyword><Keyword MajorTopicYN="N">Phytomanagement</Keyword><Keyword MajorTopicYN="N">Plant growth-promoting bacteria</Keyword><Keyword MajorTopicYN="N">Poplar habitats</Keyword><Keyword MajorTopicYN="N">Trace elements</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>09</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>12</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>6</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29890585</ArticleId><ArticleId IdType="pii">S0048-9697(17)33488-5</ArticleId><ArticleId IdType="doi">10.1016/j.scitotenv.2017.12.069</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Bourgogne-Franche-Comté</li><li>Franche-Comté</li><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Montbéliard</li><li>Vandœuvre-lès-Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="France"><region name="Bourgogne-Franche-Comté"><name sortKey="Durand, Alexis" sort="Durand, Alexis" uniqKey="Durand A" first="Alexis" last="Durand">Alexis Durand</name></region><name sortKey="Alvarez Lopez, Vanessa" sort="Alvarez Lopez, Vanessa" uniqKey="Alvarez Lopez V" first="Vanessa" last="Alvarez-Lopez">Vanessa Alvarez-Lopez</name><name sortKey="Bertheau, Coralie" sort="Bertheau, Coralie" uniqKey="Bertheau C" first="Coralie" last="Bertheau">Coralie Bertheau</name><name sortKey="Blaudez, Damien" sort="Blaudez, Damien" uniqKey="Blaudez D" first="Damien" last="Blaudez">Damien Blaudez</name><name sortKey="Chalot, Michel" sort="Chalot, Michel" uniqKey="Chalot M" first="Michel" last="Chalot">Michel Chalot</name><name sortKey="Guinchard, Sarah" sort="Guinchard, Sarah" uniqKey="Guinchard S" first="Sarah" last="Guinchard">Sarah Guinchard</name><name sortKey="Maillard, Francois" sort="Maillard, Francois" uniqKey="Maillard F" first="François" last="Maillard">François Maillard</name><name sortKey="Valot, Benoit" sort="Valot, Benoit" uniqKey="Valot B" first="Benoit" last="Valot">Benoit Valot</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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